penn addgene plasmid 44719 software (Addgene inc)
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penn addgene plasmid 44719 software
Penn Addgene Plasmid 44719 Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/44719+software/pCas9_GFP+(Plasmid+%2344719)/pm40849903-695-15-16
Average 94 stars, based on 265 article reviews
Penn Addgene Plasmid 44719 Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/44719+software/pCas9_GFP+(Plasmid+%2344719)/pm40849903-695-15-16
Average 94 stars, based on 265 article reviews
penn addgene plasmid 44719 software - by Bioz Stars,
2026-10
94/100 stars
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Recombinant:Article Title: Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APP-Y188 Abcam ab32136; RRID:AB_2289606 Tubulin Sigma T5168; RRID:AB_477579 Anti-Rabbit IgG (H+L), HRP Conjugate Promega W4011, RRID:AB_430833 Anti-Mouse IgG (H+L), HRP Conjugate Promega W4021, RRID:AB_430834 Chemicals, Peptides, and Recombinant Proteins TfiI NEB R0546S DdeI NEB R0175S XmnI NEB R0194S 2x PrimeTime Gene Expression Master Mix IDT 1055772 20x human TERT TaqMan Copy Number Reference Assay ThermoFisher 4403316 OneTaq 2x Master Mix NEB M0486L GeneRuler 100 bp Plus DNA ladder ThermoFisher SM0321 Critical Commercial Assays NucleoSpin Tissue Kit Macherey-Nagel 740952 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel 740609 TOPO TA Cloning Kit for Sequencing ThermoFisher 450030 NucleoSpin Plasmid Kit Macherey Nagel 740588 NucleoSpin RNA/Protein Kit Macherey-Nagel 740933 MSD Human (6E10) Ab V-PLEX Kit Meso Scale Discovery K15200E Experimental Models: Cell Lines 7889SA Paquet et al., 2016, NYSCF 7889SA A18944 ThermoFisher A18945 Oligonucleotides sgRNAs This paper Table S3 ssODNs for HDR-mediated editing This paper Table S3 Primers This paper Table S3 Recombinant DNA MLM3636 a gift from K. Joung, Addgene 43860 pSpCas9(BB)-2A-Puro (PX459) V2.0 a gift from F. Zhang, Addgene 62988 pCas9_GFP a gift from K. Musunuru, Gene Expression:Article Title: Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APP-Y188 Abcam ab32136; RRID:AB_2289606 Tubulin Sigma T5168; RRID:AB_477579 Anti-Rabbit IgG (H+L), HRP Conjugate Promega W4011, RRID:AB_430833 Anti-Mouse IgG (H+L), HRP Conjugate Promega W4021, RRID:AB_430834 Chemicals, Peptides, and Recombinant Proteins TfiI NEB R0546S DdeI NEB R0175S XmnI NEB R0194S 2x PrimeTime Gene Expression Master Mix IDT 1055772 20x human TERT TaqMan Copy Number Reference Assay ThermoFisher 4403316 OneTaq 2x Master Mix NEB M0486L GeneRuler 100 bp Plus DNA ladder ThermoFisher SM0321 Critical Commercial Assays NucleoSpin Tissue Kit Macherey-Nagel 740952 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel 740609 TOPO TA Cloning Kit for Sequencing ThermoFisher 450030 NucleoSpin Plasmid Kit Macherey Nagel 740588 NucleoSpin RNA/Protein Kit Macherey-Nagel 740933 MSD Human (6E10) Ab V-PLEX Kit Meso Scale Discovery K15200E Experimental Models: Cell Lines 7889SA Paquet et al., 2016, NYSCF 7889SA A18944 ThermoFisher A18945 Oligonucleotides sgRNAs This paper Table S3 ssODNs for HDR-mediated editing This paper Table S3 Primers This paper Table S3 Recombinant DNA MLM3636 a gift from K. Joung, Addgene 43860 pSpCas9(BB)-2A-Puro (PX459) V2.0 a gift from F. Zhang, Addgene 62988 pCas9_GFP a gift from K. Musunuru, Polymerase Chain Reaction:Article Title: Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APP-Y188 Abcam ab32136; RRID:AB_2289606 Tubulin Sigma T5168; RRID:AB_477579 Anti-Rabbit IgG (H+L), HRP Conjugate Promega W4011, RRID:AB_430833 Anti-Mouse IgG (H+L), HRP Conjugate Promega W4021, RRID:AB_430834 Chemicals, Peptides, and Recombinant Proteins TfiI NEB R0546S DdeI NEB R0175S XmnI NEB R0194S 2x PrimeTime Gene Expression Master Mix IDT 1055772 20x human TERT TaqMan Copy Number Reference Assay ThermoFisher 4403316 OneTaq 2x Master Mix NEB M0486L GeneRuler 100 bp Plus DNA ladder ThermoFisher SM0321 Critical Commercial Assays NucleoSpin Tissue Kit Macherey-Nagel 740952 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel 740609 TOPO TA Cloning Kit for Sequencing ThermoFisher 450030 NucleoSpin Plasmid Kit Macherey Nagel 740588 NucleoSpin RNA/Protein Kit Macherey-Nagel 740933 MSD Human (6E10) Ab V-PLEX Kit Meso Scale Discovery K15200E Experimental Models: Cell Lines 7889SA Paquet et al., 2016, NYSCF 7889SA A18944 ThermoFisher A18945 Oligonucleotides sgRNAs This paper Table S3 ssODNs for HDR-mediated editing This paper Table S3 Primers This paper Table S3 Recombinant DNA MLM3636 a gift from K. Joung, Addgene 43860 pSpCas9(BB)-2A-Puro (PX459) V2.0 a gift from F. Zhang, Addgene 62988 pCas9_GFP a gift from K. Musunuru, TA Cloning:Article Title: Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APP-Y188 Abcam ab32136; RRID:AB_2289606 Tubulin Sigma T5168; RRID:AB_477579 Anti-Rabbit IgG (H+L), HRP Conjugate Promega W4011, RRID:AB_430833 Anti-Mouse IgG (H+L), HRP Conjugate Promega W4021, RRID:AB_430834 Chemicals, Peptides, and Recombinant Proteins TfiI NEB R0546S DdeI NEB R0175S XmnI NEB R0194S 2x PrimeTime Gene Expression Master Mix IDT 1055772 20x human TERT TaqMan Copy Number Reference Assay ThermoFisher 4403316 OneTaq 2x Master Mix NEB M0486L GeneRuler 100 bp Plus DNA ladder ThermoFisher SM0321 Critical Commercial Assays NucleoSpin Tissue Kit Macherey-Nagel 740952 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel 740609 TOPO TA Cloning Kit for Sequencing ThermoFisher 450030 NucleoSpin Plasmid Kit Macherey Nagel 740588 NucleoSpin RNA/Protein Kit Macherey-Nagel 740933 MSD Human (6E10) Ab V-PLEX Kit Meso Scale Discovery K15200E Experimental Models: Cell Lines 7889SA Paquet et al., 2016, NYSCF 7889SA A18944 ThermoFisher A18945 Oligonucleotides sgRNAs This paper Table S3 ssODNs for HDR-mediated editing This paper Table S3 Primers This paper Table S3 Recombinant DNA MLM3636 a gift from K. Joung, Addgene 43860 pSpCas9(BB)-2A-Puro (PX459) V2.0 a gift from F. Zhang, Addgene 62988 pCas9_GFP a gift from K. Musunuru, Sequencing:Article Title: Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APP-Y188 Abcam ab32136; RRID:AB_2289606 Tubulin Sigma T5168; RRID:AB_477579 Anti-Rabbit IgG (H+L), HRP Conjugate Promega W4011, RRID:AB_430833 Anti-Mouse IgG (H+L), HRP Conjugate Promega W4021, RRID:AB_430834 Chemicals, Peptides, and Recombinant Proteins TfiI NEB R0546S DdeI NEB R0175S XmnI NEB R0194S 2x PrimeTime Gene Expression Master Mix IDT 1055772 20x human TERT TaqMan Copy Number Reference Assay ThermoFisher 4403316 OneTaq 2x Master Mix NEB M0486L GeneRuler 100 bp Plus DNA ladder ThermoFisher SM0321 Critical Commercial Assays NucleoSpin Tissue Kit Macherey-Nagel 740952 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel 740609 TOPO TA Cloning Kit for Sequencing ThermoFisher 450030 NucleoSpin Plasmid Kit Macherey Nagel 740588 NucleoSpin RNA/Protein Kit Macherey-Nagel 740933 MSD Human (6E10) Ab V-PLEX Kit Meso Scale Discovery K15200E Experimental Models: Cell Lines 7889SA Paquet et al., 2016, NYSCF 7889SA A18944 ThermoFisher A18945 Oligonucleotides sgRNAs This paper Table S3 ssODNs for HDR-mediated editing This paper Table S3 Primers This paper Table S3 Recombinant DNA MLM3636 a gift from K. Joung, Addgene 43860 pSpCas9(BB)-2A-Puro (PX459) V2.0 a gift from F. Zhang, Addgene 62988 pCas9_GFP a gift from K. Musunuru, Plasmid Preparation:Article Title: Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APP-Y188 Abcam ab32136; RRID:AB_2289606 Tubulin Sigma T5168; RRID:AB_477579 Anti-Rabbit IgG (H+L), HRP Conjugate Promega W4011, RRID:AB_430833 Anti-Mouse IgG (H+L), HRP Conjugate Promega W4021, RRID:AB_430834 Chemicals, Peptides, and Recombinant Proteins TfiI NEB R0546S DdeI NEB R0175S XmnI NEB R0194S 2x PrimeTime Gene Expression Master Mix IDT 1055772 20x human TERT TaqMan Copy Number Reference Assay ThermoFisher 4403316 OneTaq 2x Master Mix NEB M0486L GeneRuler 100 bp Plus DNA ladder ThermoFisher SM0321 Critical Commercial Assays NucleoSpin Tissue Kit Macherey-Nagel 740952 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel 740609 TOPO TA Cloning Kit for Sequencing ThermoFisher 450030 NucleoSpin Plasmid Kit Macherey Nagel 740588 NucleoSpin RNA/Protein Kit Macherey-Nagel 740933 MSD Human (6E10) Ab V-PLEX Kit Meso Scale Discovery K15200E Experimental Models: Cell Lines 7889SA Paquet et al., 2016, NYSCF 7889SA A18944 ThermoFisher A18945 Oligonucleotides sgRNAs This paper Table S3 ssODNs for HDR-mediated editing This paper Table S3 Primers This paper Table S3 Recombinant DNA MLM3636 a gift from K. Joung, Addgene 43860 pSpCas9(BB)-2A-Puro (PX459) V2.0 a gift from F. Zhang, Addgene 62988 pCas9_GFP a gift from K. Musunuru, Software:Article Title: Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APP-Y188 Abcam ab32136; RRID:AB_2289606 Tubulin Sigma T5168; RRID:AB_477579 Anti-Rabbit IgG (H+L), HRP Conjugate Promega W4011, RRID:AB_430833 Anti-Mouse IgG (H+L), HRP Conjugate Promega W4021, RRID:AB_430834 Chemicals, Peptides, and Recombinant Proteins TfiI NEB R0546S DdeI NEB R0175S XmnI NEB R0194S 2x PrimeTime Gene Expression Master Mix IDT 1055772 20x human TERT TaqMan Copy Number Reference Assay ThermoFisher 4403316 OneTaq 2x Master Mix NEB M0486L GeneRuler 100 bp Plus DNA ladder ThermoFisher SM0321 Critical Commercial Assays NucleoSpin Tissue Kit Macherey-Nagel 740952 NucleoSpin Gel and PCR Clean-Up Kit Macherey-Nagel 740609 TOPO TA Cloning Kit for Sequencing ThermoFisher 450030 NucleoSpin Plasmid Kit Macherey Nagel 740588 NucleoSpin RNA/Protein Kit Macherey-Nagel 740933 MSD Human (6E10) Ab V-PLEX Kit Meso Scale Discovery K15200E Experimental Models: Cell Lines 7889SA Paquet et al., 2016, NYSCF 7889SA A18944 ThermoFisher A18945 Oligonucleotides sgRNAs This paper Table S3 ssODNs for HDR-mediated editing This paper Table S3 Primers This paper Table S3 Recombinant DNA MLM3636 a gift from K. Joung, Addgene 43860 pSpCas9(BB)-2A-Puro (PX459) V2.0 a gift from F. Zhang, Addgene 62988 pCas9_GFP a gift from K. Musunuru, |